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Position: Research Assistant
Institution: University College London
Department: Great Ormond Street Institute of Child Health
Location: London, United Kingdom
Duties: The candidate is expected to assist senior staff in the development of gene therapy/gene targeting protocols enabling correction of primary immunodeficiencies. Also the post holder will be involved in the development of site specific nucleases and will optimise different delivery methods, including adeno-associated vectors (AAV), alongside with disease specific transduction protocols; Work within a team of scientists developing gene therapy and gene editing strategies, isolate and culture murine Lin negative cells from disease model or wild type mice and human CD34 cells from healthy donors or patients; The post holder will assist in the running and organization of the research laboratories, including ordering of common lab material, shelf/trolley stocking, preparation/aliquoting of lab reagents, mycoplasma testing; Duration of the post is available for 12 months in the first instance
Requirements: Candidates should have a BSc or MSc in a relevant discipline, experience in culture and manipulation of cell lines, laboratory work in molecular biology and prior knowledge and experience in techniques of flow cytometry, western blotting, cloning, PCR; Experience in culture and manipulation of stem cells, experience in gene therapy, viral production, transduction and experience in murine adoptive cell transplantation studies are desirable
   
Text: Research Assistant, - Ref:1873342 Click here to go back to search results Apply Now UCL Department / Division Great Ormond Street Institute of Child Health Location of position London Grade 6 Hours Full Time Salary (inclusive of London allowance) £31,542 - £33,257 per annum Duties and Responsibilities The candidate is expected to assist senior staff in the development of gene therapy/gene targeting protocols enabling correction of primary immunodeficiencies. Also the post holder will be involved in the development of site specific nucleases and will optimise different delivery methods, including adeno-associated vectors (AAV), alongside with disease specific transduction protocols. Work within a team of scientists developing gene therapy and gene editing strategies, isolate and culture murine Lin negative cells from disease model or wild type mice and human CD34 cells from healthy donors or patients. The post holder will assist in the running and organization of the research laboratories, including ordering of common lab material, shelf/trolley stocking, preparation/aliquoting of lab reagents, mycoplasma testing. Duration of the post is available for 12 months in the first instance. Key Requirements Candidates should have a BSc or MSc in a relevant discipline, experience in culture and manipulation of cell lines, laboratory work in molecular biology and prior knowledge and experience in techniques of flow cytometry, western blotting, cloning, PCR. Experience in culture and manipulation of stem cells, experience in gene therapy, viral production, transduction and experience in murine adoptive cell transplantation studies are desirable. Further Details A job description and person specification can be accessed at the bottom of the page. To apply for the vacancy please click on the Apply Now button below. If you have any queries regarding the application process, please contact Madhur Sharma, ICH.HR@ucl.ac.uk quoting job reference 1873342. UCL Taking Action for Equality Closing Date 21 Dec 2020 Latest time for the submission of applications 23:59 Interview date TBC Our department holds an Athena SWAN Gold award, in recognition of our long-term commitment and 'beacon' status in advancing gender equality. This appointment is subject to UCL Terms and Conditions of Service for Research and Support Staff. Please use these links to find out more about UCL working life including the benefits we offer and UCL Terms and Conditions related to this job. Job Description and Person Specification Apply Now
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