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Position: Research Assistant
Institution: University College London
Department: Department of Neurodegenerative Disease - UCL Huntingtons Disease (HD) Centre
Location: London, United Kingdom
Duties: Applications are invited for a Research Assistant to work on a Cure Huntington's Disease Initiative (CHDI) Foundation funded project within Professor Sarah J Tabrizis research group at the Huntingtons Disease Centre, UCL Queen Square Institute of Neurology; Recent genome-wide association (GWA) data from our group and others has highlighted the importance of the DNA damage response (DDR) pathway in the modulation of age at onset (AAO) and progression of Huntingtons disease (HD). The post-holder will be responsible for daily maintenance of human iPSC lines, differentiation to neuronal cultures and assisting with the day-to-day running of the tissue culture laboratory
Requirements: The successful candidate will hold Honours degree (minimum 2: 1) in biological science or a related discipline. Experience of working in experimental laboratory research is essential, as is tissue culture experience and molecular biology experience including PCR assays and transduction/transfection of cells. Experience of iPSC cultures and biochemical assays including western blotting is desirable, as is an understanding of neuroscience and neurodegenerative diseases
   
Text: Research Assistant, - Ref:1869360 Click here to go back to search results Apply Now UCL Department / Division Department of Neurodegenerative Disease Specific unit / Sub department UCL Huntingtons Disease (HD) Centre Location of position London Grade 6 Hours Full Time Salary (inclusive of London allowance) £31,479 to £33,194 pa Duties and Responsibilities Applications are invited for a Research Assistant to work on a Cure Huntington's Disease Initiative (CHDI) Foundation funded project within Professor Sarah J Tabrizis research group at the Huntingtons Disease Centre, UCL Queen Square Institute of Neurology. Recent genome-wide association (GWA) data from our group and others has highlighted the importance of the DNA damage response (DDR) pathway in the modulation of age at onset (AAO) and progression of Huntingtons disease (HD). This post will examine the involvement of DDR proteins in somatic CAG repeat expansion in the Huntingtin (HTT) gene that is observed in HD models. The post holder will use HD patient induced pluripotent stem cells (iPSCs) that demonstrate somatic instability (SI) at the CAG repeat to model the effects of DDR pathway gene mutations found in either fast or slow progressing HD patients, specifically FAN1 and MSH3 mutations. They will use techniques including antisense oligonucleotide (ASO) and shRNA-mediated knockdown, and CRISPR to manipulate expression of DDR pathway components in iPSCs, which will subsequently be differentiated to medium spiny neurons (MSNs). The effect on SI in MSNs will be monitored using CAG repeat sizing and HD phenotypes assessed using High Content Imaging (Opera Phenix). Effects on DNA repair and damage will be investigated in a number of ways including DNA repair reporter assays. The post-holder will be responsible for daily maintenance of human iPSC lines, differentiation to neuronal cultures and assisting with the day-to-day running of the tissue culture laboratory. The post is available from 1 September 2020 and is funded by a grant from the CHDI Foundation until 31 August 2022 in the first instance. Key Requirements The successful candidate will hold Honours degree (minimum 2:1) in biological science or a related discipline. Experience of working in experimental laboratory research is essential, as is tissue culture experience and molecular biology experience including PCR assays and transduction/ transfection of cells. Experience of iPSC cultures and biochemical assays including western blotting is desirable, as is an understanding of neuroscience and neurodegenerative diseases. Further Details A Job Description and Person Specification can be accessed at the bottom of this page. To apply for the vacancy, please click on the 'Apply Now' button below. Informal enquiries concerning the post can be made to Dr Michael Flower (michael.flower@ucl.ac.uk). If you have any queries regarding the application process, please contact Samantha Robinson, HR Officer, UCL Queen Square Institute of Neurology, 23 Queen Square, London, WC1N 3BG (email: ion.hradmin@ucl.ac.uk). UCL Taking Action for Equality We will consider applications to work on a part-time, flexible and job share basis wherever possible. Closing Date 30 Sep 2020 Latest time for the submission of applications 23.59 Interview date Afternoon of 9 October 2020 Our department holds an Athena SWAN Silver award, in recognition of our commitment and demonstrable impact in advancing gender equality. This appointment is subject to UCL Terms and Conditions of Service for Research and Support Staff. Please use these links to find out more about UCL working life including the benefits we offer and UCL Terms and Conditions related to this job. Job description and person specification Apply Now
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