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Position: PhD Fellowship
Institution: Institut de génétique et de biologie moléculaire et cellulaire
Location: Illkirch‐Graffenstaden, Strasbourg Eurométropole, France
Duties: The objective of this thesis is reveal the gold particles in cell sections and in cryo-ET to detect rare protein complexes such as RNA polymerase II molecules, to determine their spatial distribution and to identify their cellular interaction partners
Requirements: A graduate student with a background in physics or in cell biology would benefit from an interdisciplinary training in biological sample preparation, FIB/SEM imaging, and data interpretation. The first part of the thesis will be devoted to study of the state of the art of in situ labelling approaches, and in getting familiar with the instrumentation. A second part of the thesis will be dedicated to the characterization of newly synthetized electron-dense markers and of their behavior in a cellular environment. In a third part the student will use the most promising electron dense nanoparticles conjugated to probes recognizing RNA polymerase II
   
Text: IGBMC : Institut de la Génétique et de la Biologie Moléculaire et Cellulaire Intranet | Webmail | Contact Rechercher La recherche biomedicale l'espoir du futur ! IGBMC Recherche Technologies Formation Evénements Infrastructures Science & société 4 départements de recherche 750 employés 45 nationalités 55 équipes de recherche 16 lauréats ERC 260 publications par an 24000 m² de laboratoires Soutenez-nous via Chef d'équipe Patrick SCHULTZ patrick.schultz@igbmc.fr Tel. : +33 (0)36948 5299 Les chiffres 52 équipes de recherche 131 chercheurs 34 post-doctorants 68 doctorants 121 ingénieurs & techniciens 110 administratifs & services généraux 110 personnels ICS Accès direct Départements / Programmes Plateformes technologiques Services scientifiques PhD programme Valorisation Vie étudiante Recrutement Vivre à Strasbourg Offre d'emploi Recrutement A Phd Fellowship Is Currently Available In Our Team Within The Frame Of The Interdisciplinary Cnrs Program “80prime” Reference : PhD Subject Publication de l'offre : 25 février 2020 Important biological properties such as membrane-less compartmentalization, phase separation, condensate formation, weak or transient interaction are only occurring in the crowded environment of the intact cell. A current challenge is to identify key functional sites for transcription within the cell nucleus, and to determine their structure and molecular environment. Cryo-Electron Microscopy approaches have been developed to visualize molecular assemblies in their cellular environment1. Tri-dimensional (3-D) reconstruction of the cell is performed by cryo-Electron Tomography (cryo-ET) of thin cellular slices which are produced by cryo-sectioning the samples2 or by using a focused ion beam (FIB) to mill thin lamellae3. Cryo-ET reconstructions have revealed large molecular assemblies, such as mitochondria, ribosomes, nuclear pore complexes or cytoskeleton in molecular details1. Molecular complexes of low cellular abundance or too small to be recognized by their shape need to be labelled with an electron dense marker to be detected. To address this challenge, we synthesized gold-based probes that can be delivered into living cells. The gold nanoparticles are coupled to antibody derivatives to selectively label the target proteins. The objective of this thesis is reveal the gold particles in cell sections and in cryo-ET to detect rare protein complexes such as RNA polymerase II molecules, to determine their spatial distribution and to identify their cellular interaction partners. A graduate student with a background in physics or in cell biology would benefit from an interdisciplinary training in biological sample preparation, FIB/SEM imaging, and data interpretation. The first part of the thesis will be devoted to study of the state of the art of in situ labelling approaches, and in getting familiar with the instrumentation. A second part of the thesis will be dedicated to the characterization of newly synthetized electron-dense markers and of their behavior in a cellular environment. In a third part the student will use the most promising electron dense nanoparticles conjugated to probes recognizing RNA polymerase II. He will experience data collection on the Titan Cryo-electron microscope both in standard imaging mode and in cryo electron tomography mode. The student will conduct his research on the Illkirch campus and will share his time between the structural biology group of the IGBMC, the chemobiology group of the ESBS and the high resolution imaging group of the IPCMS. 1 Mahamid, J. et al. Visualizing the molecular sociology at the HeLa cell nuclear periphery. Science 351, 969-972 (2016). 2 Al-Amoudi, A., Studer, D. & Dubochet, J. Cutting artefacts and cutting process in vitreous sections for cryo-EM. J Struct Biol 150, 109-121 (2005). 3 Marko, M. et al. Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy. Nat Methods 4, 215-217 (2007). Votre candidature Date limite de candidature : 30 novembre 2020 Historique Investissements d'Avenir Organisation Recrutement Publications institutionnelles Prix, Distinctions & bourses d'excellence Vie étudiante Vivre à Strasbourg Plan IGBMC Historique Investissements d'Avenir Organisation Recrutement Job opportunities Publications institutionnelles Prix, distinctions & bourse d'excellence Vivre à Strasbourg Vie étudiante Plan Recherche Les équipes de l'IGBMC Dpt Biologie du développement & cellules souches Dpt Biologie structurale intégrative Dpt Génomique fonctionnelle & cancer Dpt Médecine translationnelle & neurogénétique Programme Analyses Intégratives de l’Expression des Gènes et de l’Epigénome Publications Technologies Services scientifiques Plateformes technologiques Valorisation Start-up Licences & brevets Formation PhD programme IMCBio Workshops Masters Événements Séminaires Congrès Workshops Meetings Evénements grand public Séminaires spéciaux Archives Infrastructures ICS - Institut clinique de la souris CBI - Centre de biologie intégrative Science & société Actualités scientifiques Événements grand public Prix, distinctions & bourses d'excellence Vidéos © IGBMC 2012 - Mentions légales IGBMC - CNRS UMR 7104 - Inserm U 12581 rue Laurent Fries / BP 10142 / 67404 Illkirch CEDEX / France Tél +33 (0)3 88 65 32 00 / Fax +33 (0)3 88 65 32 01 / directeur.igbmc@igbmc.fr
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